1144450Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal WatersUpdated by FeedsNodeProcessor100015119data_sourceund151361322915507868770015507868770- <p>Scottish Marine and Freshwater Science Vol 8 No 24</p>
<p>A small number of phytoplankton species have the ability to produce algal toxins which can accumulate in filter feeding bivalves such as oysters, scallops and mussels. The bioaccumulation of algal toxins can potentially cause serious health issues to shellfish consumers. Countries with a shellfish aquaculture industry (such as Scotland) have set up for their classified production areas a phytoplankton monitoring programme as part of their legal obligations (EC 854/2004 and amendments). This involves the regular collection of water samples to assess the phytoplankton community using light microscopy, with a particular emphasis on the toxin producing species. However, this technique lacks the ability to identify some key phytoplankton to a species level (e.g. Pseudo-nitzschia sp., Alexandrium sp.), which is critical to appropriately assess the potential toxicity of a phytoplankton bloom event. The objective of this project was to evaluate a microarray technique which was developed during the MIDTAL project (Microarrays For The Detection of Toxic Algae: <a href="http://www.midtal.com/">http://www.midtal.com/</a>) to identify phytoplankton to species level. Microarrays are modified glass supports on which are printed RNA probes that are species-specific. Each targeted phytoplankton species is defined by a set of probes that are statistically unique to each one. For the purpose of this project, water samples were collected offshore from Stonehaven (East coast of Scotland) over a two years period (starting in 2015) and processed using the microarray to assess its specificity and sensitivity in relation to the identification and potential semi-quantification of toxic strains of phytoplankton.</p>
full_html<p>Scottish Marine and Freshwater Science Vol 8 No 24</p>
<p>A small number of phytoplankton species have the ability to produce algal toxins which can accumulate in filter feeding bivalves such as oysters, scallops and mussels. The bioaccumulation of algal toxins can potentially cause serious health issues to shellfish consumers. Countries with a shellfish aquaculture industry (such as Scotland) have set up for their classified production areas a phytoplankton monitoring programme as part of their legal obligations (EC 854/2004 and amendments). This involves the regular collection of water samples to assess the phytoplankton community using light microscopy, with a particular emphasis on the toxin producing species. However, this technique lacks the ability to identify some key phytoplankton to a species level (e.g. Pseudo-nitzschia sp., Alexandrium sp.), which is critical to appropriately assess the potential toxicity of a phytoplankton bloom event. The objective of this project was to evaluate a microarray technique which was developed during the MIDTAL project (Microarrays For The Detection of Toxic Algae: <a href="http://www.midtal.com/">http://www.midtal.com/</a>) to identify phytoplankton to species level. Microarrays are modified glass supports on which are printed RNA probes that are species-specific. Each targeted phytoplankton species is defined by a set of probes that are statistically unique to each one. For the purpose of this project, water samples were collected offshore from Stonehaven (East coast of Scotland) over a two years period (starting in 2015) and processed using the microarray to assess its specificity and sensitivity in relation to the identification and potential semi-quantification of toxic strains of phytoplankton.</p>
- 627
- https://data.marine.gov.scot/dataset/microarray-detection-and-quantification-toxin-producing-phytoplankton-species-scottishhttps://data.marine.gov.scot/dataset/microarray-detection-and-quantification-toxin-producing-phytoplankton-species-scottish
- 696
- 728
- 1832
- 1678
- 1685
- sioc:Item
- foaf:Document
- dc:title
property- dc:date
- dc:created
xsd:dateTimedate_iso8601- dc:modified
xsd:dateTimedate_iso8601- content:encoded
property- sioc:has_creator
rel- foaf:name
- sioc:num_replies
xsd:integer- sioc:last_activity_date
xsd:dateTimedate_iso8601https://marine.gov.scot/data/microarray-detection-and-quantification-toxin-producing-phytoplankton-species-scottish-coastal10442611444515119publishedpublished0155078687711Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal Waters155078687710442611444515119publishedpublished0155078687711Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal Waters155078687710442611444515119publishedpublished0155078687711Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal Waters1550786877