{"vid":"114610","uid":"0","title":"The Development and Validation of a High Performance Liquid Chromatography Method to Quantify Chlorophylls a and b in Marine Phytoplankton and Identify Other Pigments of Interest ","log":"Updated by FeedsNodeProcessor","status":"1","comment":"1","promote":"0","sticky":"0","ds_switch":"","nid":"4215","type":"data_source","language":"und","created":"1446113979","changed":"1550786877","tnid":"0","translate":"0","revision_timestamp":"1550786877","revision_uid":"0","body":{"und":[{"value":"<p>Scottish Marine and Freshwater Science Volume 1 No 12</p>\n<ol>\n<li>\n<p>High Performance Liquid Chromatography (HPLC) is an alternative method to light  microscopy and fluorescence for characterising phytoplankton. The HPLC data  provides valuable information about the contribution of different functional groups to  the biomass of the phytoplankton community. The HPLC data should be supported  by microscopic verification as many algal classes share the same pigments.</p>\n</li>\n<li>\n<p>A quantative HPLC method was developed to quantify chlorophyll a and chlorophyll  b. However, due to a lack of commercially available standards at suitable  concentrations, only area % data is given for 19 hexanoyloxyfucoxanthin,  alloxanthin, alpha-carotene, antheraxanthin, beta-carotene, chlorophyll c2, chlorophyll  c3, diadinoxanthin, diatoxanthin, fucoxanthin, gyroxanthin-diester, lutein, neoxanthin,  peridinin, prasinoxanthin, violaxanthin and zeaxanthin.</p>\n</li>\n<li>\n<p>Ammonium acetate buffer (0.5 mol l-1) is added to the standards and samples  immediately before injection, in order to increase the affinity of the pigments for the  column during the loading step. This reaction is temperature dependant and must be  carried out at ambient temperature to ensure adequate mixing.</p>\n</li>\n<li>\n<p>The stability of the pigments, in the autosampler at ambient temperature, whilst  awaiting injection for a typical analytical run, was investigated. All pigments were  found to be stable in the autosampler for 18 hours. A typical analytical batch takes  15 hours of analytical time.</p>\n</li>\n<li>\n<p>Replicate analysis for high standards and samples and low standards and samples  on different days gave %CV of &lt;11% for both chlorophyll a concentration and  chlorophyll b concentration. This meets the limits for reproducibility given in the UK  Clean Seas Environmental Monitoring Programme (CSEMP) Green Book. 2</p>\n</li>\n<li>\n<p>The limit of detection (LOD) was calculated to be 0.1 \u00b5g l-1 for chlorophyll a and 0.02  \u00b5g l-1 for chlorophyll b. This meets the limits for LOD given in CSEMP Green Book.</p>\n</li>\n<li>\n<p>Recovery for chlorophyll a was calculated to be 105%, and 110% for chlorophyll b,  which meets the limits set by the United Kingdom National Marine Chemistry  Advisory Group (NMCAG).</p>\n</li>\n</ol>\n","summary":null,"format":"full_html","safe_value":"<p>Scottish Marine and Freshwater Science Volume 1 No 12</p>\n<ol>\n<li>\n<p>High Performance Liquid Chromatography (HPLC) is an alternative method to light  microscopy and fluorescence for characterising phytoplankton. The HPLC data  provides valuable information about the contribution of different functional groups to  the biomass of the phytoplankton community. The HPLC data should be supported  by microscopic verification as many algal classes share the same pigments.</p>\n</li>\n<li>\n<p>A quantative HPLC method was developed to quantify chlorophyll a and chlorophyll  b. However, due to a lack of commercially available standards at suitable  concentrations, only area % data is given for 19 hexanoyloxyfucoxanthin,  alloxanthin, alpha-carotene, antheraxanthin, beta-carotene, chlorophyll c2, chlorophyll  c3, diadinoxanthin, diatoxanthin, fucoxanthin, gyroxanthin-diester, lutein, neoxanthin,  peridinin, prasinoxanthin, violaxanthin and zeaxanthin.</p>\n</li>\n<li>\n<p>Ammonium acetate buffer (0.5 mol l-1) is added to the standards and samples  immediately before injection, in order to increase the affinity of the pigments for the  column during the loading step. This reaction is temperature dependant and must be  carried out at ambient temperature to ensure adequate mixing.</p>\n</li>\n<li>\n<p>The stability of the pigments, in the autosampler at ambient temperature, whilst  awaiting injection for a typical analytical run, was investigated. All pigments were  found to be stable in the autosampler for 18 hours. A typical analytical batch takes  15 hours of analytical time.</p>\n</li>\n<li>\n<p>Replicate analysis for high standards and samples and low standards and samples  on different days gave %CV of &lt;11% for both chlorophyll a concentration and  chlorophyll b concentration. This meets the limits for reproducibility given in the UK  Clean Seas Environmental Monitoring Programme (CSEMP) Green Book. 2</p>\n</li>\n<li>\n<p>The limit of detection (LOD) was calculated to be 0.1 \u00b5g l-1 for chlorophyll a and 0.02  \u00b5g l-1 for chlorophyll b. This meets the limits for LOD given in CSEMP Green Book.</p>\n</li>\n<li>\n<p>Recovery for chlorophyll a was calculated to be 105%, and 110% for chlorophyll b,  which meets the limits set by the United Kingdom National Marine Chemistry  Advisory Group (NMCAG).</p>\n</li>\n</ol>\n","safe_summary":""}]},"field_type":{"und":[{"tid":"627"}]},"field_datasource_link":{"und":[{"url":"https://data.marine.gov.scot/dataset/development-and-validation-high-performance-liquid-chromatography-method-quantify","title":null,"attributes":[],"original_title":null,"original_url":"https://data.marine.gov.scot/dataset/development-and-validation-high-performance-liquid-chromatography-method-quantify"}]},"field_citation":[],"field_reference":[],"field_map_type_tag":[],"field_datasource_keywords":{"und":[{"tid":"725"},{"tid":"726"}]},"field_tags":[],"field_manual_data_tag":[],"field_data_owner":[],"field_document":[],"field_mslot_stage":[],"field_mslot_application_type":[],"field_mslot_other_document_categ":[],"field_date_submitted":[],"field_mslot_ag_categories":[],"field_cfwg_category":[],"field_transfer_and_delegation_do":[],"rdf_mapping":{"rdftype":["sioc:Item","foaf:Document"],"title":{"predicates":["dc:title"],"type":"property"},"created":{"predicates":["dc:date","dc:created"],"datatype":"xsd:dateTime","callback":"date_iso8601"},"changed":{"predicates":["dc:modified"],"datatype":"xsd:dateTime","callback":"date_iso8601"},"body":{"predicates":["content:encoded"],"type":"property"},"uid":{"predicates":["sioc:has_creator"],"type":"rel"},"name":{"predicates":["foaf:name"]},"comment_count":{"predicates":["sioc:num_replies"],"datatype":"xsd:integer"},"last_activity":{"predicates":["sioc:last_activity_date"],"datatype":"xsd:dateTime","callback":"date_iso8601"}},"path":"https://marine.gov.scot/?q=data/development-and-validation-high-performance-liquid-chromatography-method-quantify-chlorophylls","name":"","picture":"0","data":null,"workbench_moderation":{"current":{"hid":"104591","vid":"114610","nid":"4215","from_state":"published","state":"published","uid":"0","stamp":"1550786877","published":"1","is_current":1,"title":"The Development and Validation of a High Performance Liquid Chromatography Method to Quantify Chlorophylls a and b in Marine Phytoplankton and Identify Other Pigments of Interest ","timestamp":"1550786877"},"published":{"hid":"104591","vid":"114610","nid":"4215","from_state":"published","state":"published","uid":"0","stamp":"1550786877","published":"1","is_current":1,"title":"The Development and Validation of a High Performance Liquid Chromatography Method to Quantify Chlorophylls a and b in Marine Phytoplankton and Identify Other Pigments of Interest ","timestamp":"1550786877"},"my_revision":{"hid":"104591","vid":"114610","nid":"4215","from_state":"published","state":"published","uid":"0","stamp":"1550786877","published":"1","is_current":1,"title":"The Development and Validation of a High Performance Liquid Chromatography Method to Quantify Chlorophylls a and b in Marine Phytoplankton and Identify Other Pigments of Interest ","timestamp":"1550786877"}}}